Gabapentin (GBP), a γ-aminobutyric acid iphone xr price calgary analogue used for neuropathic pain and epilepsy, can reduce cat stress and improve veterinary exam compliance.A sensitive LC-MS/MS assay was established and rigorously validated for quantifying GBP in feline plasma.The method employed acetonitrile-mediated protein precipitation to efficiently extract GBP and its internal standard, pregabalin (PGB).
Chromatographic separation was achieved within 7 min using a C18 column (2.1 × 50 mm, 1.7 μm) with a gradient mobile phase comprising 0.
1% (v/v) formic acid in water and acetonitrile.The pharmacokinetics of 25 mg/kg GBP was studied by single-dose oral and intravenous administration.The results demonstrated that the method exhibited satisfactory precision, accuracy and linearity.
The pharmacokinetic results showed that the Tmax, Cmax, T1/2 and AUC0–t of GBP in cats after oral administration of 25 mg/kg were (1.83 ± 0.75) h, (13.
94 ± 3.75) μg/mL, (5.60 ± 1.
79) h and (115.54 ± 27.56) (μg/mL) h, respectively.
The results of the study indicated that after intravenous administration of 25 mg/kg GBP, the C0, T1/2 and AUC0–t were (58.82 ± 15.34) μg/mL, (3.
87 ± 0.64) h and (160.44 read more ± 32.
65) (μg/mL) h, respectively.The oral bioavailability of GBP in cats was (78.71 ± 18.
55)%.In this study, a selective and sensitive LC-MS/MS method for the quantification of GBP in cat plasma was developed and validated.This method was successfully employed to assess the pharmacokinetics following the oral and intravenous administration of GBP in cats.